Zvi Kelman

Research Biologist

Kelman Group

Contact

Email: zkelman@umd.edu

Call: (240) 314-6294

Education

  • Ph.D., Molecular Biology, Cornell University Graduate School of Medical Sciences, New York
  • MSc., Cell Biology, Weizmann Institute of Science, Rehovot, Israel
  • BSc., Agriculture, Hebrew University of Jerusalem, Rehovot, Israel

Profile

Dr. Zvi Kelman’s research is focused on developing tools and reagents for the labeling of biomolecules with stable isotopes to support biophysical and bioanalytical measurements. The NIST Biomolecular Labeling Laboratory (BL2) also provides support to external users through a proposal process. The laboratory has the equipment and reagents necessary for production, purification, and characterization of labeled proteins and other biomolecules, including peptides and nucleic acids. A part of these efforts includes the labeling of monoclonal antibodies (mAbs).  mAbs are commonly developed by the pharmaceutical industry as drugs to fight a large variety of diseases, including cancer and autoimmune disease. Some structural studies on mAbs will benefit from the ability to label the antibodies with stable isotopes. The lab is developing tools and reagents for bacteria, yeast, plant and mammalian cell expression and purification of labeled mAbs.

Another project pursued by the lab is the development of tools and reagents for next-generation protein sequencing.  Next-gen protein sequencing is an emerging technology that shows promise to revolutionize the field of proteomics, as next-generation DNA and RNA sequencing did for the fields of genomics and transcriptomics.

Publications
2024
Engineering GID4 for use as an N-terminal proline binder via directed evolution.
An important consideration when expressing mAbs in Escherichia coli.
Extremophiles in a changing world.
2023
Effects of glycans and hinge on dynamics in the IgG1 Fc.
Contributions from ClpS surface residues in modulating N-terminal peptide binding and their implications for NAAB development.
2021
Preface.
Preface.
The emerging landscape of single-molecule protein sequencing technologies.
Recombinant expression of computationally designed peptide-bundlemers in Escherichia coli.
Creation and filtering of a recurrent spectral library of CHO cell metabolites and media components.
2020
Leveraging nature's biomolecular designs in next-generation protein sequencing reagent development.
Unwinding 20 Years of the Archaeal Minichromosome Maintenance Helicase.
2019
Strategies for Development of a Next-Generation Protein Sequencing Platform.
Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli.
Editorial: The DNA Replication Machinery as Therapeutic Targets.
Engineering ClpS for selective and enhanced N-terminal amino acid binding.
Neutron scattering in the biological sciences: progress and prospects.
2018
DNA Sliding Clamps as Therapeutic Targets.
Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli.
Heterologous recombinant expression of non-originator NISTmAb.