|Title||Coupling Self-Assembly Mechanisms to Fabricate Molecularly and Electrically Responsive Films.|
|Publication Type||Journal Article|
|Year of Publication||2019|
|Authors||Li, J, Maniar, D, Qu, X, Liu, H, Tsao, C-Y, Kim, E, Bentley, WE, Liu, C, Payne, GF|
|Date Published||2019 Jan 07|
Biomacromolecules often possess information to self-assemble through low energy competing interactions which can make self-assembly responsive to environmental cues and can also confer dynamic properties. Here we coupled self-assembling systems to create biofunctional multilayer films that can be cued to dis-assemble through either molecular or electrical signals. To create functional multi-layers we: (i) electrodeposited the pH-responsive self-assembling aminopolysaccharide chitosan; (ii) allowed the lectin Concanavalin A (ConA) to bind to the chitosan-coated electrode (presumably through electrostatic interactions); (iii) performed layer-by-layer self-assembly by sequential contacting with glycogen and ConA; and (iv) conferred biological (i.e., enzymatic) function by assembling glycoprotein (i.e., enzymes) to the ConA-terminated multilayer. Because the ConA tetramer dissociates at low pH, this multilayer can be triggered to dis-assemble by acidification. We demonstrate two approaches to induce acidification: (i) glucose oxidase can induce multilayer dis-assembly in response to molecular cues; and (ii) anodic reactions can induce multilayer dis-assembly in response to electrical cues.