

Title | Coupling Self-Assembly Mechanisms to Fabricate Molecularly and Electrically Responsive Films. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Li, J, Maniar, D, Qu, X, Liu, H, Tsao, C-Y, Kim, E, Bentley, WE, Liu, C, Payne, GF |
Journal | Biomacromolecules |
Date Published | 2019 Jan 07 |
ISSN | 1526-4602 |
Abstract | Biomacromolecules often possess information to self-assemble through low energy competing interactions which can make self-assembly responsive to environmental cues and can also confer dynamic properties. Here we coupled self-assembling systems to create biofunctional multilayer films that can be cued to dis-assemble through either molecular or electrical signals. To create functional multi-layers we: (i) electrodeposited the pH-responsive self-assembling aminopolysaccharide chitosan; (ii) allowed the lectin Concanavalin A (ConA) to bind to the chitosan-coated electrode (presumably through electrostatic interactions); (iii) performed layer-by-layer self-assembly by sequential contacting with glycogen and ConA; and (iv) conferred biological (i.e., enzymatic) function by assembling glycoprotein (i.e., enzymes) to the ConA-terminated multilayer. Because the ConA tetramer dissociates at low pH, this multilayer can be triggered to dis-assemble by acidification. We demonstrate two approaches to induce acidification: (i) glucose oxidase can induce multilayer dis-assembly in response to molecular cues; and (ii) anodic reactions can induce multilayer dis-assembly in response to electrical cues. |
DOI | 10.1021/acs.biomac.8b01592 |
Alternate Journal | Biomacromolecules |
PubMed ID | 30616349 |