An immune magnetic nano-assembly for specifically amplifying intercellular quorum sensing signals.

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TitleAn immune magnetic nano-assembly for specifically amplifying intercellular quorum sensing signals.
Publication TypeJournal Article
Year of Publication2018
AuthorsGuan, Y, Tsao, C-Y, Quan, DN, Li, Y, Mei, L, Zhang, J, Zhang, B, Liu, Y, Bentley, WE, Payne, GF, Wang, Q
JournalColloids Surf B Biointerfaces
Volume172
Pagination197-206
Date Published2018 Aug 18
ISSN1873-4367
Abstract

Quorum sensing (QS) enables intercellular communication after bacterial cells sense the autoinducers have reached or exceeded a critical concentration. Selectively amplifying specific bacterial "quorum" activity at a lower cell density is still a challenge. Here, we propose a novel platform of immune magnetic nano-assembly to amplify specific bacterial QS signaling via improving the bioavailability of autoinducers-2 (AI-2, furanosyl borate) from sender (wide-type, WT cells) to receiver (reporter cells). Antibody coated magnetic nanoparticle (MNPAB) was fabricated with an average diameter of 12 nm and a specific surface area of 96.5 m/g. The distribution efficiency of the antibody on the surface was 25.8 μg/m of magnetic nanoparticles. It was found that more than 3 × 10 of K12 serotype Escherichia coli (E. coli) reporter or WT cells were collected using 1 mg fabricated MNPAB at a saturated condition. The MNPAB not only captured E. coli WT cells but also brought them into proximity of E. coli (CT104, pCT6+pET-DsRed) reporter cells via magnetic attraction. The amplified QS signaling of the reporter cells by this immune magnetic nano-assembly was approximately 3 times higher than the nature QS signaling in cell suspension at optical density (OD) 0.08. This study foresees potential applications in amplifying specific biological QS signals based on a preprogrammed design.

DOI10.1016/j.colsurfb.2018.08.033
Alternate JournalColloids Surf B Biointerfaces
PubMed ID30170233