The Splicing Factor Mediates the Cellular Immune Response in .

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TitleThe Splicing Factor Mediates the Cellular Immune Response in .
Publication TypeJournal Article
Year of Publication2018
AuthorsNazario-Toole, AE, Robalino, J, Okrah, K, Corrada-Bravo, H, Mount, SM, Wu, LP
JournalJ Immunol
Volume201
Issue4
Pagination1154-1164
Date Published2018 Aug 15
ISSN1550-6606
Abstract

The uptake and destruction of bacteria by phagocytic cells is an essential defense mechanism in metazoans. To identify novel genes involved in the phagocytosis of , a major human pathogen, we assessed the phagocytic capacity of adult blood cells (hemocytes) of the fruit fly, by testing several lines of the Drosophila Genetic Reference Panel. Natural genetic variation in the gene () correlated with low phagocytic capacity in hemocytes, pointing to Rbfox1 as a candidate regulator of phagocytosis. Loss of Rbfox1 resulted in increased expression of the Ig superfamily member (). Silencing of in Rbfox1-depleted blood cells rescued the fly's cellular immune response to , indicating that downregulation of by Rbfox1 is critical for phagocytosis in To our knowledge, this study is the first to demonstrate a link between and host defense against .

DOI10.4049/jimmunol.1800496
Alternate JournalJ. Immunol.
PubMed ID29997126
PubMed Central IDPMC6085876
Grant ListT32 AI089621 / AI / NIAID NIH HHS / United States
T32 GM080201 / GM / NIGMS NIH HHS / United States