|Title||Focusing quorum sensing signaling by nano-magnetic assembly.|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Guan, Y, Tsao, C-Y, Quan, DN, Li, Y, Mei, L, Song, Y, Zhang, B, Liu, Y, Payne, GF, Bentley, WE, Wang, Q|
|Date Published||2018 May 28|
Quorum sensing (QS) exists widely among bacteria, enabling a transition to multicellular behavior after bacterial populations reach a particular density. The coordination of multicellularity enables biotechnological application, dissolution of biofilms, coordination of virulence, etc. Here, a method to elicit and subsequently disperse multi-cellular behavior among QS-negative cells is developed using magnetic nanoparticle assembly. We fabricated magnetic nanoparticles (MNPs, ∼5 nm) that electrostatically collect wild-type (WT) Escherichia coli BL21 cells and brings them into proximity of bioengineered E. coli (CT104 (W3110 lsrFG luxS pCT6+pET-DsRed)) reporter cells that exhibit a QS response after receiving autoinducer-2 (AI-2). By shortening the distance between WT and reporter cells (e.g., increasing local available AI-2 concentrations), the QS response signaling was amplified 4-fold compared to that in native conditions without assembly. This study suggests potential applications in facilitating intercellular communication and modulating multicellular behaviors based on user-specified designs. This article is protected by copyright. All rights reserved.
|Alternate Journal||Environ. Microbiol.|
|Grant List||R21 EB024102 / EB / NIBIB NIH HHS / United States|