Focusing quorum sensing signaling by nano-magnetic assembly.

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TitleFocusing quorum sensing signaling by nano-magnetic assembly.
Publication TypeJournal Article
Year of Publication2018
AuthorsGuan, Y, Tsao, C-Y, Quan, DN, Li, Y, Mei, L, Song, Y, Zhang, B, Liu, Y, Payne, GF, Bentley, WE, Wang, Q
JournalEnviron Microbiol
Date Published2018 May 28
ISSN1462-2920
Abstract

Quorum sensing (QS) exists widely among bacteria, enabling a transition to multicellular behavior after bacterial populations reach a particular density. The coordination of multicellularity enables biotechnological application, dissolution of biofilms, coordination of virulence, etc. Here, a method to elicit and subsequently disperse multi-cellular behavior among QS-negative cells is developed using magnetic nanoparticle assembly. We fabricated magnetic nanoparticles (MNPs, ∼5 nm) that electrostatically collect wild-type (WT) Escherichia coli BL21 cells and brings them into proximity of bioengineered E. coli (CT104 (W3110 lsrFG luxS pCT6+pET-DsRed)) reporter cells that exhibit a QS response after receiving autoinducer-2 (AI-2). By shortening the distance between WT and reporter cells (e.g., increasing local available AI-2 concentrations), the QS response signaling was amplified 4-fold compared to that in native conditions without assembly. This study suggests potential applications in facilitating intercellular communication and modulating multicellular behaviors based on user-specified designs. This article is protected by copyright. All rights reserved.

DOI10.1111/1462-2920.14284
Alternate JournalEnviron. Microbiol.
PubMed ID29806719
Grant ListR21 EB024102 / EB / NIBIB NIH HHS / United States