Evidence of link between quorum sensing and sugar metabolism in revealed via cocrystal structures of LsrK and HPr.

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TitleEvidence of link between quorum sensing and sugar metabolism in revealed via cocrystal structures of LsrK and HPr.
Publication TypeJournal Article
Year of Publication2018
AuthorsHa, J-H, Hauk, P, Cho, K, Eo, Y, Ma, X, Stephens, K, Cha, S, Jeong, M, Suh, J-Y, Sintim, HO, Bentley, WE, Ryu, K-S
JournalSci Adv
Volume4
Issue6
Paginationeaar7063
Date Published2018 Jun
ISSN2375-2548
Abstract

Quorum sensing (QS), a bacterial process that regulates population-scale behavior, is mediated by small signaling molecules, called autoinducers (AIs), that are secreted and perceived, modulating a "collective" phenotype. Because the autoinducer AI-2 is secreted by a wide variety of bacterial species, its "perception" cues bacterial behavior. This response is mediated by the (LuxS-regulated) operon that includes the AI-2 transporter LsrACDB and the kinase LsrK. We report that HPr, a phosphocarrier protein central to the sugar phosphotransferase system of , copurifies with LsrK. Cocrystal structures of an LsrK/HPr complex were determined, and the effects of HPr and phosphorylated HPr on LsrK activity were assessed. LsrK activity is inhibited when bound to HPr, revealing new linkages between QS activity and sugar metabolism. These findings help shed new light on the abilities of bacteria to rapidly respond to changing nutrient levels at the population scale. They also suggest new means of manipulating QS activity among bacteria and within various niches.

DOI10.1126/sciadv.aar7063
Alternate JournalSci Adv
PubMed ID29868643
PubMed Central IDPMC5983913