Catechol-chitosan redox capacitor for added amplification in electrochemical immunoanalysis.

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TitleCatechol-chitosan redox capacitor for added amplification in electrochemical immunoanalysis.
Publication TypeJournal Article
Year of Publication2018
AuthorsYan, K, Liu, Y, Guan, Y, Bhokisham, N, Tsao, C-Y, Kim, E, Shi, X-W, Wang, Q, Bentley, WE, Payne, GF
JournalColloids Surf B Biointerfaces
Volume169
Pagination470-477
Date Published2018 May 22
ISSN1873-4367
Abstract

Antibodies are common recognition elements for molecular detection but often the signals generated by their stoichiometric binding must be amplified to enhance sensitivity. Here, we report that an electrode coated with a catechol-chitosan redox capacitor can amplify the electrochemical signal generated from an alkaline phosphatase (AP) linked immunoassay. Specifically, the AP product p-aminophenol (PAP) undergoes redox-cycling in the redox capacitor to generate amplified oxidation currents. We estimate an 8-fold amplification associated with this redox-cycling in the capacitor (compared to detection by a bare electrode). Importantly, this capacitor-based amplification is generic and can be coupled to existing amplification approaches based on enzyme-linked catalysis or magnetic nanoparticle-based collection/concentration. Thus, the capacitor should enhance sensitivities in conventional immunoassays and also provide chemical to electrical signal transduction for emerging applications in molecular communication.

DOI10.1016/j.colsurfb.2018.05.048
Alternate JournalColloids Surf B Biointerfaces
PubMed ID29852436