Two-Way Chemical Communication between Artificial and Natural Cells.

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TitleTwo-Way Chemical Communication between Artificial and Natural Cells.
Publication TypeJournal Article
Year of Publication2017
AuthorsLentini, R, Martín, NYeh, Forlin, M, Belmonte, L, Fontana, J, Cornella, M, Martini, L, Tamburini, S, Bentley, WE, Jousson, O, Mansy, SS
JournalACS Cent Sci
Volume3
Issue2
Pagination117-123
Date Published2017 Feb 22
ISSN2374-7943
Abstract

Artificial cells capable of both sensing and sending chemical messages to bacteria have yet to be built. Here we show that artificial cells that are able to sense and synthesize quorum signaling molecules can chemically communicate with V. fischeri, V. harveyi, E. coli, and P. aeruginosa. Activity was assessed by fluorescence, luminescence, RT-qPCR, and RNA-seq. Two potential applications for this technology were demonstrated. First, the extent to which artificial cells could imitate natural cells was quantified by a type of cellular Turing test. Artificial cells capable of sensing and in response synthesizing and releasing N-3-(oxohexanoyl)homoserine lactone showed a high degree of likeness to natural V. fischeri under specific test conditions. Second, artificial cells that sensed V. fischeri and in response degraded a quorum signaling molecule of P. aeruginosa (N-(3-oxododecanoyl)homoserine lactone) were constructed, laying the foundation for future technologies that control complex networks of natural cells.

DOI10.1021/acscentsci.6b00330
Alternate JournalACS Cent Sci
PubMed ID28280778
PubMed Central IDPMC5324081