Redox Probing for Chemical Information of Oxidative Stress.

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TitleRedox Probing for Chemical Information of Oxidative Stress.
Publication TypeJournal Article
Year of Publication2016
AuthorsKim, E, Winkler, TE, Kitchen, C, Kang, M, Banis, G, Bentley, WE, Kelly, DL, Ghodssi, R, Payne, GF
JournalAnal Chem
Date Published2016 Dec 29
ISSN1520-6882
Abstract

Oxidative stress is implicated in many diseases yet no simple, rapid and robust measurement is available at the point-of-care to assist clinicians in detecting oxidative stress. Here, we report results from a discovery-based research approach in which a redox mediator is used to probe serum samples for chemical information relevant to oxidative stress. Specifically, we use an iridium salt (K2IrCl6) to probe serum for reducing activities that can transfer electrons to iridium and thus generate detectable optical and electrochemical signals. We show that this Ir-reducing assay can detect various biological reductants and is especially sensitive to glutathione (GSH) compared to alternative assays. We performed an initial clinical evaluation using serum from 10 people diagnosed with schizophrenia, a mental health disorder that is increasingly linked to oxidative stress. The measured Ir-reducing capacity was able to discriminate people with schizophrenia from healthy controls (p < 0.005) and correlations were observed between Ir-reducing capacity and independent measures of symptom severity.

DOI10.1021/acs.analchem.6b03620
Alternate JournalAnal. Chem.
PubMed ID28035805