|Title||Anti-biofilm activities of a novel chimeolysin against S. mutans in physiological and cariogenic conditions.|
|Publication Type||Journal Article|
|Year of Publication||2016|
|Authors||Yang, H, Bi, Y, Shang, X, Wang, M, Linden, SB, Li, Y, Li, Y, Nelson, DC, Wei, H|
|Journal||Antimicrob Agents Chemother|
|Date Published||2016 Oct 10|
Streptococcus mutans often survives as a biofilm on the tooth surface and contributes to the development of dental caries. We investigated the efficacy of ClyR, an engineered chimeolysin, against S. mutans biofilms in physiological and cariogenic conditions. Susceptibility tests showed that ClyR was active against all clinical S. mutans isolates tested as well as S. mutans biofilms that displayed resistance to penicillin. S. mutans biofilms formed on hydroxyapatite discs in physiological sugar conditions and cariogenic conditions were reduced ∼2 logs and 3 logs after treatment with 100 μg/ml ClyR, respectively. In comparison, only 1 log reduction was observed in the chlorhexidine (ChX) treated group, and no killing effect was observed in the NaF treated group. A mouse dental colonization model showed that repeated use of ClyR for 3 weeks (5 μg/day) reduced the number of colonized S. mutans in the dental plaques significantly (p<0.05), without harmful effects on mice. Furthermore, toxicity was not noted at concentrations exceeding those used for these studies and ClyR-specific antibodies could not be detected in mice saliva after repeated use of ClyR in the oral cavity. Our data collectively demonstrates that ClyR is active against S. mutans biofilms both in vitro and in vivo, thus representing a preventative or therapeutic agent against dental caries.
|Alternate Journal||Antimicrob. Agents Chemother.|