Data on biochemical fluxes generated from biofabricated enzyme complexes assembled through engineered tags and microbial transglutaminase.

Printer-friendly versionPrinter-friendly versionPDF versionPDF version
TitleData on biochemical fluxes generated from biofabricated enzyme complexes assembled through engineered tags and microbial transglutaminase.
Publication TypeJournal Article
Year of Publication2016
AuthorsBhokisham, N, Pakhchanian, H, Quan, D, Tschirhart, T, Tsao, C-Y, Payne, GF, Bentley, WE
JournalData Brief
Volume8
Pagination1031-5
Date Published2016 Sep
ISSN2352-3409
Abstract

Data presented is related to an article titled "Modular construction of multi-subunit protein complexes using engineered tags and microbial transglutaminase" (Bhokisham et al., 2016) [1]. In this article, we have presented western blot and flux data associated with assembly of Pfs-LuxS enzyme complexes on beads using uni-tagged and bi-tagged LuxS enzymes. We have also presented biochemical flux following changes in enzyme stoichiometries. We covalently coupled a Pfs-LuxS complex with Protein G, an antibody binding non-enzyme component and directed these complexes to the surfaces of bacterial cells via anti-Escherichia coli antibodies. Fluorescence microscopy images represented the altered behavior of bacterial cells in response to the autoinducer-2 that is synthesized by the Protein G-enzyme complexes.

DOI10.1016/j.dib.2016.07.005
Alternate JournalData Brief
PubMed ID27508259
PubMed Central IDPMC4969242