Glycoprotein analysis using mass spectrometry: unraveling the layers of complexity.

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TitleGlycoprotein analysis using mass spectrometry: unraveling the layers of complexity.
Publication TypeJournal Article
Year of Publication2012
AuthorsSchiel, JE
JournalAnal Bioanal Chem
Volume404
Issue4
Pagination1141-9
Date Published2012 Sep
ISSN1618-2650
KeywordsAmino Acid Sequence, Animals, Glycoproteins, Glycosylation, Humans, Mass Spectrometry, Molecular Sequence Data
Abstract

A glycoprotein exists as a heterogeneous mixture of forms due to differential glycosylation, each of which may confer different functionality and/or serve as a biochemical marker for disease. The complex structure of glycans make them a bioanalytical challenge requiring multiple mass spectrometry based approaches to gain different types of information. The following article will briefly describe recently utilized mass spectrometry methods to identify glycosylation sites and measure glycan composition, sequence, branching, and relative quantities. Potential metrological developments are discussed in light of current trends toward complete, reliable glycoanalytical characterization in a high-throughput manner.

DOI10.1007/s00216-012-6185-2
Alternate JournalAnal Bioanal Chem
PubMed ID22733248