QUANTITY: An Isobaric Tag for Quantitative Glycomics.

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TitleQUANTITY: An Isobaric Tag for Quantitative Glycomics.
Publication TypeJournal Article
Year of Publication2015
AuthorsYang, S, Wang, M, Chen, L, Yin, B, Song, G, Turko, IV, Phinney, KW, Betenbaugh, MJ, Zhang, H, Li, S
JournalSci Rep
Volume5
Pagination17585
Date Published2015
ISSN2045-2322
Abstract<p>Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics - analysis of glycans at global level - however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As a result, technologies that can facilitate global glycan analysis are highly sought after. Here, we present QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that can not only enhance detection of glycans by mass spectrometry, but also allow high-throughput glycomic analysis from multiple biological samples. This robust tool enabled us to accomplish glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells with knock-in/out enzymes involved in protein glycosylation. Our results demonstrated QUANTITY is an invaluable technique for glycan analysis and bioengineering.</p>
DOI10.1038/srep17585
Alternate JournalSci Rep
PubMed ID26616285
PubMed Central IDPMC4663469