A chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method.

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TitleA chimeolysin with extended-spectrum streptococcal host range found by an induced lysis-based rapid screening method.
Publication TypeJournal Article
Year of Publication2015
AuthorsYang, H, Linden, SB, Wang, J, Yu, J, Nelson, DC, Wei, H
JournalSci Rep
Volume5
Pagination17257
Date Published2015
ISSN2045-2322
Abstract<p>The increasing emergence of multi-drug resistant streptococci poses a serious threat to public health worldwide. Bacteriophage lysins are promising alternatives to antibiotics; however, their narrow lytic spectrum restricted to closely related species is a central shortcoming to their translational development. Here, we describe an efficient method for rapid screening of engineered chimeric lysins and report a unique "chimeolysin", ClyR, with robust activity and an extended-spectrum streptococcal host range against most streptococcal species, including S. pyogenes, S. agalactiae, S. dysgalactiae, S. equi, S. mutans, S. pneumoniae, S. suis and S. uberis, as well as representative enterococcal and staphylococcal species (including MRSA and VISA). ClyR is the first lysin that demonstrates activity against the dominant dental caries-causing pathogen as well as the first lysin that kills all four of the bovine mastitis-causing pathogens. This study demonstrates the success of the screening method resulting in a powerful lysin with potential for treating most streptococcal associated infections.</p>
DOI10.1038/srep17257
Alternate JournalSci Rep
PubMed ID26607832
PubMed Central IDPMC4660466