Measurement of homonuclear proton couplings from regular 2D COSY spectra.

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TitleMeasurement of homonuclear proton couplings from regular 2D COSY spectra.
Publication TypeJournal Article
Year of Publication2001
AuthorsDelaglio, F, Wu, Z, Bax, A
JournalJ Magn Reson
Volume149
Issue2
Pagination276-81
Date Published2001 Apr
ISSN1090-7807
KeywordsComputer Graphics, Fourier Analysis, Humans, Magnetic Resonance Spectroscopy, Protons, Signal Processing, Computer-Assisted, Software, Ubiquitins
Abstract

An interactive computer procedure is described which determines (1)H--(1)H couplings from fitting the cross-peak multiplets in regular phase-sensitive COSY spectra. The robustness and simplicity of the method rely on the fact that a given cross-peak intensity is not an independent variable in the fitting procedure, making it possible to measure couplings accurately even from individual cross peaks with unresolved multiplet structure.

DOI10.1006/jmre.2001.2297
Alternate JournalJ. Magn. Reson.
PubMed ID11318630