Solution structure of (gamma)S-crystallin by molecular fragment replacement NMR.

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TitleSolution structure of (gamma)S-crystallin by molecular fragment replacement NMR.
Publication TypeJournal Article
Year of Publication2005
AuthorsWu, Z, Delaglio, F, Wyatt, K, Wistow, G, Bax, A
JournalProtein Sci
Volume14
Issue12
Pagination3101-14
Date Published2005 Dec
ISSN0961-8368
KeywordsAnimals, Databases, Protein, gamma-Crystallins, Mice, Models, Molecular, Nuclear Magnetic Resonance, Biomolecular, Peptide Fragments, Protein Structure, Tertiary, Reproducibility of Results
Abstract

The solution structure of murine gammaS-crystallin (gammaS) has been determined by multidimensional triple resonance NMR spectroscopy, using restraints derived from two sets of dipolar couplings, recorded in different alignment media, and supplemented by a small number of NOE distance restraints. gammaS consists of two topologically similar domains, arranged with an approximate twofold symmetry, and each domain shows close structural homology to closely related (approximately 50% sequence identity) domains found in other members of the gamma-crystallin family. Each domain consists of two four-strand "Greek key" beta-sheets. Although the domains are tightly anchored to one another by the hydrophobic surfaces of the two inner Greek key motifs, the N-arm, the interdomain linker and several turn regions show unexpected flexibility and disorder in solution. This may contribute entropic stabilization to the protein in solution, but may also indicate nucleation sites for unfolding or other structural transitions. The method used for solving the gammaS structure relies on the recently introduced molecular fragment replacement method, which capitalizes on the large database of protein structures previously solved by X-ray crystallography and NMR.

DOI10.1110/ps.051635205
Alternate JournalProtein Sci.
PubMed ID16260758
PubMed Central IDPMC2253246