Nano-guided cell networks as conveyors of molecular communication.

Printer-friendly versionPrinter-friendly versionPDF versionPDF version
TitleNano-guided cell networks as conveyors of molecular communication.
Publication TypeJournal Article
Year of Publication2015
AuthorsTerrell, JL, Wu, H-C, Tsao, C-Y, Barber, NB, Servinsky, MD, Payne, GF, Bentley, WE
JournalNat Commun
Volume6
Pagination8500
Date Published2015
ISSN2041-1723
Abstract

Advances in nanotechnology have provided unprecedented physical means to sample molecular space. Living cells provide additional capability in that they identify molecules within complex environments and actuate function. We have merged cells with nanotechnology for an integrated molecular processing network. Here we show that an engineered cell consortium autonomously generates feedback to chemical cues. Moreover, abiotic components are readily assembled onto cells, enabling amplified and 'binned' responses. Specifically, engineered cell populations are triggered by a quorum sensing (QS) signal molecule, autoinducer-2, to express surface-displayed fusions consisting of a fluorescent marker and an affinity peptide. The latter provides means for attaching magnetic nanoparticles to fluorescently activated subpopulations for coalescence into colour-indexed output. The resultant nano-guided cell network assesses QS activity and conveys molecular information as a 'bio-litmus' in a manner read by simple optical means.

DOI10.1038/ncomms9500
Alternate JournalNat Commun
PubMed ID26455828
PubMed Central IDPMC4633717