Targeting gene expression to the female larval fat body of transgenic Aedes aegypti mosquitoes.

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TitleTargeting gene expression to the female larval fat body of transgenic Aedes aegypti mosquitoes.
Publication TypeJournal Article
Year of Publication2013
AuthorsTotten, DC, Vuong, M, Litvinova, OV, Jinwal, UK, Gulia-Nuss, M, Harrell, RA, Beneš, H
JournalInsect Mol Biol
Volume22
Issue1
Pagination18-30
Date Published2013 Feb
ISSN1365-2583
Keywords5' Flanking Region, Aedes, Animals, Animals, Genetically Modified, beta-Galactosidase, Enhancer Elements, Genetic, Escherichia coli, Fat Body, Female, Gene Expression Regulation, Insect Proteins, Larva, Male, Promoter Regions, Genetic, Regulatory Sequences, Nucleic Acid
Abstract

As the fat body is a critical tissue for mosquito development, metamorphosis, immune and reproductive system function, the characterization of regulatory modules targeting gene expression to the female mosquito fat body at distinct life stages is much needed for multiple, varied strategies for controlling vector-borne diseases such as dengue and malaria. The hexameric storage protein, Hexamerin-1.2, of the mosquito Aedes atropalpus is female-specific and uniquely expressed in the fat body of fourth instar larvae and young adults. We have identified in the Hex-1.2 gene, a short regulatory module that directs female-, tissue-, and stage-specific lacZ reporter gene expression using a heterologous promoter in transgenic lines of the dengue vector Aedes aegypti. Male transgenic larvae and pupae of one line expressed no Escherichia coli β-galactosidase or transgene product; in two other lines reporter gene activity was highly female-biased. All transgenic lines expressed the reporter only in the fat body; however, lacZ mRNA levels were no different in males and females at any stage examined, suggesting that the gene regulatory module drives female-specific expression by post-transcriptional regulation in the heterologous mosquito. This regulatory element from the Hex-1.2 gene thus provides a new molecular tool for transgenic mosquito control as well as functional genetic analysis in aedine mosquitoes.

DOI10.1111/imb.12005
Alternate JournalInsect Mol. Biol.
PubMed ID23241066
PubMed Central IDPMC4101173
Grant ListAI046738 / AI / NIAID NIH HHS / United States
R01 AI046738 / AI / NIAID NIH HHS / United States
R21 AI094168 / AI / NIAID NIH HHS / United States