Modulation of RNA Polymerase II Phosphorylation Downstream of Pathogen Perception Orchestrates Plant Immunity.

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TitleModulation of RNA Polymerase II Phosphorylation Downstream of Pathogen Perception Orchestrates Plant Immunity.
Publication TypeJournal Article
Year of Publication2014
AuthorsLi, F, Cheng, C, Cui, F, de Oliveira, MVV, Yu, X, Meng, X, Intorne, AC, Babilonia, K, Li, M, Li, B, Chen, S, Ma, X, Xiao, S, Zheng, Y, Fei, Z, Metz, RP, Johnson, CD, Koiwa, H, Sun, W, Li, Z, Filho, GA de Souza, Shan, L, He, P
JournalCell Host Microbe
Volume16
Issue6
Pagination748-58
Date Published2014 Dec 10
ISSN1934-6069
Abstract<p>Perception of microbe-associated molecular patterns (MAMPs) elicits host transcriptional reprogramming as part of the immune response. Although pathogen perception is well studied, the signaling networks orchestrating immune gene expression remain less clear. In a genetic screen for components involved in the early immune gene transcription reprogramming, we identified Arabidopsis RNA polymerase II C-terminal domain (CTD) phosphatase-like 3 (CPL3) as a negative regulator of immune gene expression. MAMP perception induced rapid and transient cyclin-dependent kinase C (CDKC)-mediated phosphorylation of Arabidopsis CTD. The CDKCs, which are in turn phosphorylated and activated by a canonical MAP kinase (MAPK) cascade, represent a point of signaling convergence downstream of multiple immune receptors. CPL3 directly dephosphorylated CTD to counteract MAPK-mediated CDKC regulation. Thus, modulation of the phosphorylation dynamics of eukaryotic RNA polymerase II transcription machinery by MAPKs, CTD kinases, and phosphatases constitutes an essential mechanism for rapid orchestration of host immune gene expression and defense upon pathogen attacks.</p>
DOI10.1016/j.chom.2014.10.018
Alternate JournalCell Host Microbe
PubMed ID25464831
PubMed Central IDPMC4268009
Grant ListR01 GM092893 / GM / NIGMS NIH HHS / United States
R01 GM097247 / GM / NIGMS NIH HHS / United States