Fabrication of biomimetically patterned surfaces and their application to probing plant-bacteria interactions.

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TitleFabrication of biomimetically patterned surfaces and their application to probing plant-bacteria interactions.
Publication TypeJournal Article
Year of Publication2014
AuthorsZhang, B, Luo, Y, Pearlstein, AJ, Aplin, J, Liu, Y, Bauchan, GR, Payne, GF, Wang, Q, Nou, X, Millner, PD
JournalACS Appl Mater Interfaces
Volume6
Issue15
Pagination12467-78
Date Published2014 Aug 13
ISSN1944-8252
Abstract<p>We have developed a two-step replica molding method for rapid fabrication of biomimetically patterned plant surfaces (BPS) using polydimethylsiloxane (PDMS-BPS) and agarose (AGAR-BPS). Beyond providing multiple identical specimens that faithfully reproduce leaf surface microstructure, this approach also offers unique chemical, physical, and biological features. PDMS-BPS provide good structural durability for SEM examination, have surface wettability comparable to plant surfaces for coating development, and allow for real-time monitoring of biosynthesis through incorporation into microfluidic devices. AGAR-BPS are compatible with bacterial growth, recovery, and quantification, and enable investigation of the effects of surface topography on spatially varying survival and inactivation of Escherichia coli cells during biocide treatment. Further development and application of these biomimetically patterned surfaces to study (and possibly modify) other aspects of plant-bacteria interactions can provide insight into controlling pathogen contamination in a wide range of applications.</p>
DOI10.1021/am502384q
Alternate JournalACS Appl Mater Interfaces
PubMed ID25007271