Poly[di(sodium carboxylatoethylphenoxy)phosphazene] (PCEP) is a potent enhancer of mixed Th1/Th2 immune responses in mice immunized with influenza virus antigens.

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TitlePoly[di(sodium carboxylatoethylphenoxy)phosphazene] (PCEP) is a potent enhancer of mixed Th1/Th2 immune responses in mice immunized with influenza virus antigens.
Publication TypeJournal Article
Year of Publication2007
AuthorsMutwiri, G, Benjamin, P, Soita, H, Townsend, H, Yost, R, Roberts, B, Andrianov, AK, Babiuk, LA
JournalVaccine
Volume25
Issue7
Pagination1204-13
Date Published2007 Jan 26
ISSN0264-410X
KeywordsAdjuvants, Immunologic, Alum Compounds, Animals, Antibodies, Viral, Antibody Specificity, Antigens, Viral, Cytokines, Dose-Response Relationship, Immunologic, Enzyme-Linked Immunosorbent Assay, Immunity, Cellular, Immunoglobulin G, Influenza A Virus, H3N2 Subtype, Influenza Vaccines, Mice, Mice, Inbred BALB C, Neutralization Tests, Phenylpropionates, Polymers, Serum Albumin, Bovine, Spleen, Th1 Cells, Th2 Cells
Abstract

We investigated the ability of a novel polyphosphazene polyelectrolyte, poly[di(sodium carboxylatoethylphenoxy)phosphazene] (PCEP) to enhance antigen-specific immune responses. BALB/c mice were immunized once subcutaneously with either bovine serum albumin (BSA) or influenza virus X:31 antigen alone, or in combination with PCEP, or either of the adjuvants poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) and alum. Both PCEP and PCPP significantly enhanced serum antigen-specific total IgG, IgG1 and IgG2a antibody titers, and these responses were highest in PCEP-immunized mice. Alum induced only a modest enhancement of antibody responses. Reducing the dose of X:31 antigen by 25-fold had no effect on antibody responses in mice immunized with PCPP and PCEP, but resulted in reduced titers in those immunized with alum. Analysis of X:31 antigen-specific cytokines revealed that alum and PCPP were associated with a predominantly IL-4 response. In contrast, PCEP was associated with production of both IFNgamma and IL-4. We conclude that PCEP is a potent enhancer of antigen-specific Th1 and Th2 immune responses and is a promising adjuvant for vaccine applications.

DOI10.1016/j.vaccine.2006.10.011
Alternate JournalVaccine
PubMed ID17140708