Optimization for a recombinant E. coli fed-batch fermentation.

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TitleOptimization for a recombinant E. coli fed-batch fermentation.
Publication TypeJournal Article
Year of Publication1995
AuthorsChen, Q, Bentley, WE, Weigand, WA
JournalAppl Biochem Biotechnol
Volume51-52
Pagination449-61
Date Published1995 Spring
ISSN0273-2289
KeywordsAlgorithms, Biomass, Computer Simulation, Escherichia coli, Fermentation, Gene Dosage, Kinetics, Models, Biological, Recombinant Fusion Proteins, Software, Time Factors
Abstract

The operating strategy that produces the maximum foreign protein expression for a fed-batch process is desired. This is achieved by using a feasible quadratic programming (FSQP) algorithm with a structured model that describes cell growth and product formation for recombinant E. coli. Optimization calculations for a fed-batch culture have not been performed with a model of this complexity up to this point. A constraint on the maximum cell concentration was included. For a fixed value of batch time, the results show that the optimal time profile of feed flow rate can increase the yield of foreign protein by 12-29% over a constant feed rate policy. Also, it was found that the computation time for the FSQP algorithm can be reduced significantly by considering suboptimal profiles of the feed rate, with a minor effect on calculated protein yield.

Alternate JournalAppl. Biochem. Biotechnol.
PubMed ID7668849