Mutagenesis of putative acylation sites alters function, localization, and accumulation of a Gi alpha subunit of the chestnut blight fungus Cryphonectria parasitica.

Printer-friendly versionPrinter-friendly versionPDF versionPDF version
TitleMutagenesis of putative acylation sites alters function, localization, and accumulation of a Gi alpha subunit of the chestnut blight fungus Cryphonectria parasitica.
Publication TypeJournal Article
Year of Publication1998
AuthorsGao, S, Nuss, DL
JournalMol Plant Microbe Interact
Volume11
Issue11
Pagination1130-5
Date Published1998 Nov
ISSN0894-0282
KeywordsAcylation, Ascomycota, Base Sequence, DNA Primers, Genetic Complementation Test, GTP-Binding Protein alpha Subunits, Gi-Go, Mutagenesis, Site-Directed, Phenotype, Signal Transduction
Abstract

Targeted disruption of cpg-1, a gene encoding the G protein Gi alpha subunit, CPG-1, in the chestnut blight fungus, Cryphonectria parasitica, results in reduced mycelial growth, reduced orange pigmentation, loss of virulence, loss of asexual sporulation, and female infertility. We report the development of a complementation system for cpg-1 null mutants and its use to evaluate the in vivo consequences of mutating conserved putative CPG-1 myristoylation (G2) and palmitoylation (C3) sites. Independent mutations of the two putative acylation sites differentially altered complex fungal biological processes, including virulence, and modified CPG-1 membrane association. Results of combined Northern (RNA) and Western (immunoblot) analysis also indicated a role for lipid modification in post-transcriptional regulation of CPG-1 accumulation.

DOI10.1094/MPMI.1998.11.11.1130
Alternate JournalMol. Plant Microbe Interact.
PubMed ID9805400
Grant ListGM55981 / GM / NIGMS NIH HHS / United States