A novel endocytic pathway induced by clustering endothelial ICAM-1 or PECAM-1.

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TitleA novel endocytic pathway induced by clustering endothelial ICAM-1 or PECAM-1.
Publication TypeJournal Article
Year of Publication2003
AuthorsMuro, S, Wiewrodt, R, Thomas, A, Koniaris, L, Albelda, SM, Muzykantov, VR, Koval, M
JournalJ Cell Sci
Volume116
IssuePt 8
Pagination1599-609
Date Published2003 Apr 15
ISSN0021-9533
KeywordsAmides, Amiloride, Antibodies, Monoclonal, Antigens, CD31, Caveolin 1, Caveolins, Cell Line, Tumor, Cells, Cultured, Clathrin, Dynamins, Endocytosis, Endothelium, Vascular, Enzyme Inhibitors, Humans, Intercellular Adhesion Molecule-1, Lactones, Macrolides, Models, Biological, Protein Binding, Protein Kinase C, Pyridines, Thiazoles, Time Factors
Abstract

Antibody conjugates directed against intercellular adhesion molecule (ICAM-1) or platelet-endothelial cell adhesion molecule (PECAM-1) have formed the basis for drug delivery vehicles that are specifically recognized and internalized by endothelial cells. There is increasing evidence that ICAM-1 and PECAM-1 may also play a role in cell scavenger functions and pathogen entry. To define the mechanisms that regulate ICAM-1 and PECAM-1 internalization, we examined the uptake of anti-PECAM-1 and anti-ICAM-1 conjugates by endothelial cells. We found that the conjugates must be multimeric, because monomeric anti-ICAM-1 and anti-PECAM-1 are not internalized. Newly internalized anti-ICAM-1 and anti-PECAM-1 conjugates did not colocalize with either clathrin or caveolin, and immunoconjugate internalization was not reduced by inhibitors of clathrin-mediated or caveolar endocytosis, suggesting that this is a novel endocytic pathway. Amiloride and protein kinase C (PKC) inhibitors, agents known to inhibit macropinocytosis, reduced the internalization of clustered ICAM-1 and PECAM-1. However, expression of dominant-negative dynamin-2 constructs inhibited uptake of clustered ICAM-1. Binding of anti-ICAM-1 conjugates stimulated the formation of actin stress fibers by human umbilical vein endothelial cells (HUVEC). Latrunculin, radicicol and Y27632 also inhibited internalization of clustered ICAM-1, suggesting that actin rearrangements requiring Src kinase and Rho kinase (ROCK) were required for internalization. Interestingly, these kinases are part of the signal transduction pathways that are activated when circulating leukocytes engage endothelial cell adhesion molecules, suggesting the possibility that CAM-mediated endocytosis is regulated using comparable signaling pathways.

Alternate JournalJ. Cell. Sci.
PubMed ID12640043
Grant ListHL60290 / HL / NHLBI NIH HHS / United States