Post-integration behavior of a Mos1 mariner gene vector in Aedes aegypti.

Printer-friendly versionPrinter-friendly versionPDF versionPDF version
TitlePost-integration behavior of a Mos1 mariner gene vector in Aedes aegypti.
Publication TypeJournal Article
Year of Publication2003
AuthorsWilson, R, Orsetti, J, Klocko, AD, Aluvihare, C, Peckham, E, Atkinson, PW, Lehane, MJ, O'Brochta, DA
JournalInsect Biochem Mol Biol
Volume33
Issue9
Pagination853-63
Date Published2003 Sep
ISSN0965-1748
KeywordsAedes, Animals, Animals, Genetically Modified, Base Sequence, Blotting, Southern, DNA Transposable Elements, DNA-Binding Proteins, Genes, Insect, Genetic Vectors, Germ Cells, Mitosis, Phenotype, Recombination, Genetic, Transposases
Abstract

The post-integration behavior of insect gene vectors will determine the types of applications for which they can be used. Transposon mutagenesis, enhancer trapping, and the use of transposable elements as genetic drive systems in insects requires transposable elements with high rates of remobilization in the presence of transposase. We investigated the post-integration behavior of the Mos1 mariner element in transgenic Aedes aegypti by examining both germ-line and somatic transpositions of a non-autonomous element in the presence of Mos1 transposase. Somatic transpositions were occasionally detected while germ-line transposition was only rarely observed. Only a single germ-line transposition event was recovered after screening 14,000 progeny. The observed patterns of transposition suggest that Mos1 movement takes place between the S phase and anaphase. The data reported here indicate that Mos1 will be a useful vector in Ae. aegypti for applications requiring a very high degree of vector stability but will have limited use in the construction of genetic drive, enhancer trap, or transposon tagging systems in this species.

Alternate JournalInsect Biochem. Mol. Biol.
PubMed ID12915177
Grant ListAI 45743 / AI / NIAID NIH HHS / United States