Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli.

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TitleCyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli.
Publication TypeJournal Article
Year of Publication2005
AuthorsWang, L, Hashimoto, Y, Tsao, C-Y, Valdes, JJ, Bentley, WE
JournalJ Bacteriol
Volume187
Issue6
Pagination2066-76
Date Published2005 Mar
ISSN0021-9193
KeywordsBacterial Proteins, Carbon-Sulfur Lyases, Cyclic AMP, Cyclic AMP Receptor Protein, Escherichia coli, Escherichia coli Proteins, Fermentation, Gene Expression Regulation, Bacterial, Glucose, Homoserine, Lactones, Mutagenesis, N-Glycosyl Hydrolases, Operon, Promoter Regions, Genetic, Receptors, Cell Surface, Receptors, Cyclic AMP, Transcription Factors
Abstract

Bacterial autoinducer 2 (AI-2) is proposed to be an interspecies mediator of cell-cell communication that enables cells to operate at the multicellular level. Many environmental stimuli have been shown to affect the extracellular AI-2 levels, carbon sources being among the most important. In this report, we show that both AI-2 synthesis and uptake in Escherichia coli are subject to catabolite repression through the cyclic AMP (cAMP)-CRP complex, which directly stimulates transcription of the lsr (for "luxS regulated") operon and indirectly represses luxS expression. Specifically, cAMP-CRP is shown to bind to a CRP binding site located in the upstream region of the lsr promoter and works with the LsrR repressor to regulate AI-2 uptake. The functions of the lsr operon and its regulators, LsrR and LsrK, previously reported in Salmonella enterica serovar Typhimurium, are confirmed here for E. coli. The elucidation of cAMP-CRP involvement in E. coli autoinduction impacts many areas, including the growth of E. coli in fermentation processes.

DOI10.1128/JB.187.6.2066-2076.2005
Alternate JournalJ. Bacteriol.
PubMed ID15743955
PubMed Central IDPMC1064054