Patterned assembly of genetically modified viral nanotemplates via nucleic acid hybridization.

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TitlePatterned assembly of genetically modified viral nanotemplates via nucleic acid hybridization.
Publication TypeJournal Article
Year of Publication2005
AuthorsYi, H, Nisar, S, Lee, S-Y, Powers, MA, Bentley, WE, Payne, GF, Ghodssi, R, Rubloff, GW, Harris, MT, Culver, JN
JournalNano Lett
Volume5
Issue10
Pagination1931-6
Date Published2005 Oct
ISSN1530-6984
KeywordsAmino Acid Substitution, Capsid Proteins, Chitosan, Cysteine, DNA Probes, Microscopy, Electron, Mutation, Nanostructures, Nucleic Acid Hybridization, Ribonucleases, RNA, Viral, Tobacco Mosaic Virus, Virion
Abstract

The patterning of nanoparticles represents a significant obstacle in the assembly of nanoscale materials and devices. In this report, cysteine residues were genetically engineered onto the virion surface of tobacco mosaic virus (TMV), providing attachment sites for fluorescent markers. To pattern these viruses, labeled virions were partially disassembled to expose 5' end RNA sequences and hybridized to virus-specific probe DNA linked to electrodeposited chitosan. Electron microscopy and RNAase treatments confirmed the patterned assembly of the virus templates onto the chitosan surface. These findings demonstrate that TMV nanotemplates can be dimensionally assembled via nucleic acid hybridization.

DOI10.1021/nl051254r
Alternate JournalNano Lett.
PubMed ID16218712