Chitosan-mediated in situ biomolecule assembly in completely packaged microfluidic devices.

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TitleChitosan-mediated in situ biomolecule assembly in completely packaged microfluidic devices.
Publication TypeJournal Article
Year of Publication2006
AuthorsPark, JJin, Luo, X, Yi, H, Valentine, TM, Payne, GF, Bentley, WE, Ghodssi, R, Rubloff, GW
JournalLab Chip
Volume6
Issue10
Pagination1315-21
Date Published2006 Oct
ISSN1473-0197
KeywordsBiosensing Techniques, Chitosan, Electrochemistry, Electrodes, Gold, Microfluidic Analytical Techniques
Abstract

We report facile in situ biomolecule assembly at readily addressable sites in microfluidic channels after complete fabrication and packaging of the microfluidic device. Aminopolysaccharide chitosan's pH responsive and chemically reactive properties allow electric signal-guided biomolecule assembly onto conductive inorganic surfaces from the aqueous environment, preserving the activity of the biomolecules. A transparent and nonpermanently packaged device allows consistently leak-free sealing, simple in situ and ex situ examination of the assembly procedures, fluidic input/outputs for transport of aqueous solutions, and electrical ports to guide the assembly onto the patterned gold electrode sites within the channel. Both in situ fluorescence and ex situ profilometer results confirm chitosan-mediated in situ biomolecule assembly, demonstrating a simple approach to direct the assembly of biological components into a completely fabricated device. We believe that this strategy holds significant potential as a simple and generic biomolecule assembly approach for future applications in complex biomolecular or biosensing analyses as well as in sophisticated microfluidic networks as anticipated for future lab-on-a-chip devices.

DOI10.1039/b603101c
Alternate JournalLab Chip
PubMed ID17102845