|Title||TMV microarrays: hybridization-based assembly of DNA-programmed viral nanotemplates.|
|Publication Type||Journal Article|
|Year of Publication||2007|
|Authors||Yi, H, Rubloff, GW, Culver, JN|
|Date Published||2007 Feb 27|
|Keywords||Base Sequence, Centrifugation, Density Gradient, DNA, Genes, Viral, Genome, Viral, Microscopy, Electron, Scanning, Molecular Sequence Data, Nanoparticles, Nanotechnology, Nucleic Acid Conformation, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis, Sucrose, Tobacco Mosaic Virus|
The intrinsic ability of biological molecules to self-organize into complex structures has the potential to revolutionize methods for the assembly of nanomaterials and devices. In this work, nucleic acid hybridization was used to simultaneously assemble different Tobacco mosaic virus (TMV) nanotemplates onto a glass substrate patterned with address specific capture DNAs. To accomplish this, TMV-based nanotemplates were programmed with linker DNAs containing sequence specific addresses and hybridized directly to the capture DNAs. This assembly process proved to be a reliable, selective, and controllable means to assemble multiple TMV nanotemplates.