|Title||Chitosan biotinylation and electrodeposition for selective protein assembly.|
|Publication Type||Journal Article|
|Year of Publication||2008|
|Authors||Shi, X-W, Liu, Y, Lewandowski, AT, Wu, L-Q, Wu, H-C, Ghodssi, R, Rubloff, GW, Bentley, WE, Payne, GF|
|Date Published||2008 May 13|
|Keywords||Antibodies, Avidin, Biotinylation, Chitosan, Electroplating, Proteins, Staphylococcal Protein A|
An alternative route to protein assembly at surfaces based on using the unique capabilities of biological materials for the spatially selective assembly of proteins is described. Specifically, the stimuli-responsive properties of aminopolysaccharide chitosan are combined with the molecular-recognition capabilities of biotin-streptavidin binding. Biotinylated chitosan retains its stimuli-responsive properties and is capable of electrodepositing at specific electrode addresses. Once deposited, it is capable of binding streptavidin, which can mediate the subsequent assembly of biotinylated proteins. Spatially selective protein assembly using biotinylated Protein A and fluorescently-labeled antibodies is demonstrated.
|Alternate Journal||Macromol Biosci|