Chitosan biotinylation and electrodeposition for selective protein assembly.

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TitleChitosan biotinylation and electrodeposition for selective protein assembly.
Publication TypeJournal Article
Year of Publication2008
AuthorsShi, X-W, Liu, Y, Lewandowski, AT, Wu, L-Q, Wu, H-C, Ghodssi, R, Rubloff, GW, Bentley, WE, Payne, GF
JournalMacromol Biosci
Volume8
Issue5
Pagination451-7
Date Published2008 May 13
ISSN1616-5195
KeywordsAntibodies, Avidin, Biotinylation, Chitosan, Electroplating, Proteins, Staphylococcal Protein A
Abstract

An alternative route to protein assembly at surfaces based on using the unique capabilities of biological materials for the spatially selective assembly of proteins is described. Specifically, the stimuli-responsive properties of aminopolysaccharide chitosan are combined with the molecular-recognition capabilities of biotin-streptavidin binding. Biotinylated chitosan retains its stimuli-responsive properties and is capable of electrodepositing at specific electrode addresses. Once deposited, it is capable of binding streptavidin, which can mediate the subsequent assembly of biotinylated proteins. Spatially selective protein assembly using biotinylated Protein A and fluorescently-labeled antibodies is demonstrated.

DOI10.1002/mabi.200700220
Alternate JournalMacromol Biosci
PubMed ID18213592