Oxygen microscopy by two-photon-excited phosphorescence.

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TitleOxygen microscopy by two-photon-excited phosphorescence.
Publication TypeJournal Article
Year of Publication2008
AuthorsFinikova, OS, Lebedev, AY, Aprelev, A, Troxler, T, Gao, F, Garnacho, C, Muro, S, Hochstrasser, RM, Vinogradov, SA
Date Published2008 Aug 25
KeywordsCells, Cultured, Endothelial Cells, Humans, Luminescent Measurements, Microscopy, Molecular Structure, Nanostructures, Oxygen, Photochemistry, Photons

High-resolution images of oxygen distributions in microheterogeneous samples are obtained by two-photon laser scanning microscopy (2P LSM), using a newly developed dendritic nanoprobe with internally enhanced two-photon absorption (2PA) cross-section. In this probe, energy is harvested by a 2PA antenna, which passes excitation onto a phosphorescent metalloporphyrin via intramolecular energy transfer. The 2P LSM allows sectioning of oxygen gradients with near diffraction-limited resolution, and lifetime-based acquisition eliminates dependence on the local probe concentration. The technique is validated on objects with a priori known oxygen distributions and applied to imaging of pO(2) in cells.

Alternate JournalChemphyschem
PubMed ID18663708
PubMed Central IDPMC2645351
Grant ListEB007279 / EB / NIBIB NIH HHS / United States
HL081273 / HL / NHLBI NIH HHS / United States
P41-RR001348 / RR / NCRR NIH HHS / United States
R01 EB007279-02 / EB / NIBIB NIH HHS / United States