Beyond silencing--engineering applications of RNA interference and antisense technology for altering cellular phenotype.

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TitleBeyond silencing--engineering applications of RNA interference and antisense technology for altering cellular phenotype.
Publication TypeJournal Article
Year of Publication2008
AuthorsHebert, CG, Valdes, JJ, Bentley, WE
JournalCurr Opin Biotechnol
Volume19
Issue5
Pagination500-5
Date Published2008 Oct
ISSN0958-1669
KeywordsBiotechnology, Cell Physiological Phenomena, Gene Silencing, Genetic Engineering, Metabolomics, Phenotype, RNA Interference, RNA, Antisense
Abstract

Since its discovery 10 years ago, RNA interference (RNAi) has evolved from a research tool into a powerful method for altering the phenotype of cells and whole organisms. Its near universal applicability coupled with its pinpoint accuracy for suppressing target proteins has altered the landscape of many fields. While there is considerable intellectual investment in therapeutics, its potential extends far beyond. In this review, we explore some of these emerging applications--metabolic engineering for enhancing recombinant protein production in both insect and mammalian cell systems, antisense technologies in bacteria as next generation antibiotics, and RNAi in plant biotechnology for improving productivity and nutritional value.

DOI10.1016/j.copbio.2008.08.006
Alternate JournalCurr. Opin. Biotechnol.
PubMed ID18760358
Grant ListGM70851-01 / GM / NIGMS NIH HHS / United States