

Title | Arabidopsis 14-3-3 lambda is a positive regulator of RPW8-mediated disease resistance. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Authors | Yang, X, Wang, W, Coleman, M, Orgil, U, Feng, J, Ma, X, Ferl, R, Turner, JG, Xiao, S |
Journal | Plant J |
Volume | 60 |
Issue | 3 |
Pagination | 539-50 |
Date Published | 2009 Nov |
ISSN | 1365-313X |
Keywords | 14-3-3 Proteins, Arabidopsis, Arabidopsis Proteins, Cell Death, Gene Expression Regulation, Plant, Plant Diseases, Protein Binding, Salicylic Acid |
Abstract | The RPW8 locus from Arabidopsis thaliana Ms-0 includes two functional paralogous genes (RPW8.1 and RPW8.2) and confers broad-spectrum resistance via the salicylic acid-dependent signaling pathway to the biotrophic fungal pathogens Golovinomyces spp. that cause powdery mildew diseases on multiple plant species. To identify proteins involved in regulation of the RPW8 protein function, a yeast two-hybrid screen was performed using RPW8.2 as bait. The 14-3-3 isoform lambda (designated GF14lambda) was identified as a potential RPW8.2 interactor. The RPW8.2-GF14lambda interaction was specific and engaged the C-terminal domain of RPW8.2, which was confirmed by pulldown assays. The physiological impact of the interaction was revealed by knocking down GF14lambda by T-DNA insertion, which compromised basal and RPW8-mediated resistance to powdery mildew. In addition, over-expression of GF14lambda resulted in hypersensitive response-like cell death and enhanced resistance to powdery mildew via the salicylic acid-dependent signaling pathway. The results from this study suggest that GF14lambda may positively regulate the RPW8.2 resistance function and play a role in enhancing basal resistance in Arabidopsis. |
DOI | 10.1111/j.1365-313X.2009.03978.x |
Alternate Journal | Plant J. |
PubMed ID | 19624472 |
Grant List | 83/P15679 / / Biotechnology and Biological Sciences Research Council / United Kingdom |