A new lipid anchor for sparsely tethered bilayer lipid membranes.

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TitleA new lipid anchor for sparsely tethered bilayer lipid membranes.
Publication TypeJournal Article
Year of Publication2009
AuthorsHeinrich, F, Ng, T, Vanderah, DJ, Shekhar, P, Mihailescu, M, Nanda, H, Lösche, M
JournalLangmuir
Volume25
Issue7
Pagination4219-29
Date Published2009 Apr 7
ISSN0743-7463
KeywordsCell Membrane, Glycerol, Glyceryl Ethers, Lipid Bilayers
Abstract

Mixed self-assembled monolayers (SAMs) of beta-mercaptoethanol and the new synthetic lipid 1,2-dipalmityl-3-[w-mercaptonona(ethylene oxide)] glycerol (FC 16) were investigated for their ability to form sparsely tethered bilayer lipid membranes (stBLMs) completed with various phospholipids. We investigated the structural and functional properties of FC16-based stBLMs and compared these to stBLMs prepared using a previously characterized synthetic lipid, 1,2-dimyristyl-3-[omega-mercaptohexa(ethylene oxide)] glycerol (WC14). FC16-based stBLMs show increased resistivity to ion transfer and an increase in the submembrane space of approximately 0.5 nm. Importantly, FC16-based stBLMs formed well-defined, complete bilayers with charged phospholipids such as 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG). In these, POPG incorporates into the outer monolayer leaflet in the same ratio as in the immersion solution but is excluded from the inner leaflet. In all cases that we have investigated thus far, the area densities of the lipids within the bilayers were on average close to those in free bilayer membranes. For charged phospholipids, FC16 appears to provide a distinct advantage over WC14 for the formation of well-defined stBLMs.

Alternate JournalLangmuir
PubMed ID19714901