Detecting protein interactions in live cells via complementation of a hydrolysis-deficient β-lactamase.

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TitleDetecting protein interactions in live cells via complementation of a hydrolysis-deficient β-lactamase.
Publication TypeJournal Article
Year of Publication2011
AuthorsJiang, J, Zhang, J, Li, S
JournalChem Commun (Camb)
Volume47
Issue1
Pagination182-4
Date Published2011 Jan 7
ISSN1364-548X
Keywordsbeta-Lactamases, Biocatalysis, Cells, Cultured, HeLa Cells, Humans, Hydrolysis, Molecular Conformation, Protein Binding, Protein Interaction Mapping, Proteins
Abstract

Protein-fragment complementation assay using a hydrolysis-deficient β-lactamase provides a robust tool for studying protein-protein interactions.

DOI10.1039/c0cc01998d
Alternate JournalChem. Commun. (Camb.)
PubMed ID20697643