|Title||Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation.|
|Publication Type||Journal Article|
|Year of Publication||2011|
|Authors||Cheng, Y, Luo, X, Tsao, C-Y, Wu, H-C, Betz, J, Payne, GF, Bentley, WE, Rubloff, GW|
|Date Published||2011 Jul 21|
|Keywords||Alginates, Animals, Cell Line, Electrochemical Techniques, Electrodes, Escherichia coli, Gels, Glucuronic Acid, Hexuronic Acids, Hydrogen-Ion Concentration, Luminescent Proteins, Mice, Microfluidic Analytical Techniques|
Programmable 3D cell assembly under physiological pH conditions is achieved using electrodeposited stimuli-responsive alginate gels in a microfluidic device, with parallel sidewall electrodes enabling direct observation of the cell assembly. Electrically triggered assembly and subsequent viability of mammalian cells is demonstrated, along with spatially programmable, multi-address assembly of different strains of E. coli cells. Our approach enables in vitro study of dynamic cellular and inter-cellular processes, from cell growth and stimulus/response to inter-colony and inter-species signaling.
|Alternate Journal||Lab Chip|