Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation.

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TitleBiocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation.
Publication TypeJournal Article
Year of Publication2011
AuthorsCheng, Y, Luo, X, Tsao, C-Y, Wu, H-C, Betz, J, Payne, GF, Bentley, WE, Rubloff, GW
JournalLab Chip
Volume11
Issue14
Pagination2316-8
Date Published2011 Jul 21
ISSN1473-0189
KeywordsAlginates, Animals, Cell Line, Electrochemical Techniques, Electrodes, Escherichia coli, Gels, Glucuronic Acid, Hexuronic Acids, Hydrogen-Ion Concentration, Luminescent Proteins, Mice, Microfluidic Analytical Techniques
Abstract

Programmable 3D cell assembly under physiological pH conditions is achieved using electrodeposited stimuli-responsive alginate gels in a microfluidic device, with parallel sidewall electrodes enabling direct observation of the cell assembly. Electrically triggered assembly and subsequent viability of mammalian cells is demonstrated, along with spatially programmable, multi-address assembly of different strains of E. coli cells. Our approach enables in vitro study of dynamic cellular and inter-cellular processes, from cell growth and stimulus/response to inter-colony and inter-species signaling.

DOI10.1039/c1lc20306a
Alternate JournalLab Chip
PubMed ID21629950