

Title | Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Cheng, Y, Luo, X, Tsao, C-Y, Wu, H-C, Betz, J, Payne, GF, Bentley, WE, Rubloff, GW |
Journal | Lab Chip |
Volume | 11 |
Issue | 14 |
Pagination | 2316-8 |
Date Published | 2011 Jul 21 |
ISSN | 1473-0189 |
Keywords | Alginates, Animals, Cell Line, Electrochemical Techniques, Electrodes, Escherichia coli, Gels, Glucuronic Acid, Hexuronic Acids, Hydrogen-Ion Concentration, Luminescent Proteins, Mice, Microfluidic Analytical Techniques |
Abstract | Programmable 3D cell assembly under physiological pH conditions is achieved using electrodeposited stimuli-responsive alginate gels in a microfluidic device, with parallel sidewall electrodes enabling direct observation of the cell assembly. Electrically triggered assembly and subsequent viability of mammalian cells is demonstrated, along with spatially programmable, multi-address assembly of different strains of E. coli cells. Our approach enables in vitro study of dynamic cellular and inter-cellular processes, from cell growth and stimulus/response to inter-colony and inter-species signaling. |
DOI | 10.1039/c1lc20306a |
Alternate Journal | Lab Chip |
PubMed ID | 21629950 |