Effects on membrane lateral pressure suggest permeation mechanisms for bacterial quorum signaling molecules.

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TitleEffects on membrane lateral pressure suggest permeation mechanisms for bacterial quorum signaling molecules.
Publication TypeJournal Article
Year of Publication2011
AuthorsKamaraju, K, Smith, J, Wang, J, Roy, V, Sintim, HO, Bentley, WE, Sukharev, S
JournalBiochemistry
Volume50
Issue32
Pagination6983-93
Date Published2011 Aug 16
ISSN1520-4995
KeywordsCell Membrane Permeability, Escherichia coli, Quorum Sensing, Surface Tension
Abstract

Quorum sensing is an intricate example of "social" behavior in microbial communities mediated by small secreted molecules (autoinducers). The mechanisms of membrane permeation remain elusive for many of them. Here we present the assessment of membrane permeability for three natural autoinducers and four synthetic analogues based on their polarity, surface activity, affinity for lipid monolayers, and ability to induce lateral pressure changes in the inner E. coli membrane sensed by the bacterial tension-activated channel MscS. AI-1 (N-(3-oxodecanoyl)-l-homoserine lactone) is surface-active, and it robustly inserts into lipid monolayers, indicating strong propensity toward membranes. When presented to membrane patches from the cytoplasmic side, AI-1 transiently shifts MscS's activation curve toward higher tensions due to intercalation into the cytoplasmic leaflet followed by redistribution to the opposite side. Indole showed no detectable surface activity at the air-water interface but produced a moderate increase of lateral pressure in monolayers and was potent at shifting activation curves of MscS, demonstrating transients on sequential additions. AI-2 (4,5-dihydroxy-2,3-pentanedione, DPD) showed little activity at the interfaces, correspondingly with no effect on MscS activation. After chemical modification with isobutyl, hexyl, or heptyl chains, AI-2 displayed strong surface activity. Hexyl and especially heptyl AI-2 induced robust transient shifts of MscS activation curves. The data strongly suggest that both AI-1 and indole are directly permeable through the membrane. AI-2, more hydrophilic, shows low affinity toward lipids and thus requires a transport system, whereas alkyl analogues of AI-2 should permeate the membrane directly.

DOI10.1021/bi200684z
Alternate JournalBiochemistry
PubMed ID21736305
PubMed Central IDPMC3163381
Grant ListGM075225 / GM / NIGMS NIH HHS / United States
R01 GM075225-01A1 / GM / NIGMS NIH HHS / United States