NIST BMD/IBBR Seminar: E-GADS: We’ve created an Electrospray Glycopeptide Abundance Distribution Spectrum

Motivated by interest in the complex glycan shield on the SARS-CoV-2 Spike protein, we have undertaken a program to develop mass spectral libraries to aid in its characterization. We first extended our existing library and software to represent complex glycopeptides and then discovered an effective way to represent the site-specific distributions of N-glycans on any protein, including the 22 sites of the Spike. The evolution, future and applications of this program will be discussed.