NIST BMD/IBBR Seminar: “New Technologies to Study Archaeal DNA Replication and Repair”

DNA replication and repair are essential biological processes needed for the survival of all organisms across all domains of life. Interestingly, a variety of archaeal species thrive in extreme environments, including high temperature, high salt, and low pH, and must maintain their genome under such extremes.  In this talk I will present two technologies developed and utilized by our lab at New England Biolabs to study DNA replication and repair in hyperthermophilic Archaea.  The first, RADAR-seq: RAre DAmage and Repair Sequencing, is a Next Generation Sequencing technology that identifies and detects DNA damage on a genome-wide scale. RADAR-seq can be adapted to study a variety of biological processes including DNA replication, Restriction-Modification systems, and CRISPR-Cas. The second technology is a high-throughput functional genomics screen to identify novel DNA repair and replication enzymes from hyperthermophilic Archaea.  Likewise, the functional genomics screen can be adopted to identify a wide-variety of novel enzymes from single and metagenomic samples.

Meeting link:

https://umd.webex.com/umd/j.php?MTID=m1a492c223973a0e8d39a1c4a306b5a45

Meeting number:

120 051 1478

Password:

PHnEYy4W2d2