Cell Imaging

Biomolecular spectroscopy and imaging

The IBBR houses a number of molecular spectroscopy instruments (e.g. FTIR, CD, Confocal, SPR, EIS) that allow measurement of the structure and interactions of biological molecules, complexes formed by these molecules and heterogeneous mixtures of these molecules in solution.

Confocal imaging

The Zeiss LSM 710 confocal microscope has excitation laser lines at 405, 458, 488, 514, 561 and 633nm. It is has 34 separate spectral detection channels and is capable of detecting many standard fluorophores, including DAPI, CFP, GFP, fluorescein, Rhodamine, Texas Red, RFP, CY5 and several of the AlexFluor dyes. Suitable applications include imaging of single, double and triple-labeled specimens, 3-dimensional image analysis, colocalization studies, immunofluorescence, time series and FRAP experiments. A separate workstation with a copy of the Zeiss LSM software is available for image analysis and processing.

IVIS Lumina XR imaging system

A Lumina XR in vivo imaging system (IVIS) is available for imaging rodents, plants, flow cells, or any other system that requires real-time imaging. The system enables noninvasive visualization of cellular and genetic activity within a living system. Optical imaging allows the detection of both bioluminescent and fluorescent illuminating sources within an organism by tagging specific proteins within cells or organisms by a gene encoding one of the luciferase enzymes or fluorescent protein that enable bacteria, insects, and animals to emit light at defined wavelengths.  Fluorescence allows not only reporter-based approaches, but the ability to target specific molecules within the living organism with the use of fluorescent probes. This enables a non-invasive approach to observe biological functions, the spread of disease and/or the effects of a drug throughout an organism. The system also incorporates planar X-ray equipment that enables automated X-ray co-registration to optical images.